Here the mutations are shown to limit the binding of RNA polymerase to the core promoter sequence. On the contrary, mutations that destroy the conserveness of the consensus resulting in poor transcription efficiency are known as down mutations. Splice junctions often found between exon-intron boundaries are also considered as consensus sequences.Īny mutations or alterations in the bases of a core promoter sequence, which makes it a stronger promoter in inducing the RNA polymerase activity for efficient transcription, are known as up mutations. Transposons also recognize their target sequences and function based on the consensus sequences. Restriction enzymes also have consensus sequences in the form of palindromic repeats that specify the site for the action of enzymes for digestion. Transcription factors are the best examples to understand the consensus sequences where they recognize particular patterns in the promoters of their regulatory genes. Got a question on this topic?Ī protein binding site on a gene, often represented by a consensus sequence is a short sequence of nucleotides and is found several times in the genome and is thought to play the same role at different locations. Conserved sequences are represented by using certain notations and by graphical representations. Such sites are used to predict gene elements such as promoter, enhancer, UTR regions. Bioinformatic tools such as JalView and UGENE are used to calculate and predict consensus sequences. The conserved and specificity nature of these regulatory functions are found to be conserved over long periods of evolution and hence are used to delineate the evolutionary relatedness based on the amount of conservation in these sites. A protein binding site on a gene, often represented by a consensus sequence is a short sequence of nucleotides and is found several times in the genome and is thought to play the same role at different locations. It is calculated as the most frequently appearing residue in the nucleotide or amino acid sequences. It is a model for a putative DNA binding site that is obtained by aligning all the known examples of a specific recognition site. The consensus sequence is defined as an idealized sequence that represents the predominant base at each position.
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